Dr. Dongyan Zhang Adjunct Assistant Professor PhD, Osaka University Molecular Biology, Biotechnology, and Bio-nanotechnology Phone: 906.487.2086 Fax: 906.487.2933 Email: dozhang@mtu.edu

Professional Biography Appointments and Honors Professional Preparation and Experience Selected Publications

Professional Biography

Dr. Zhang has received exceptional training on microbiology, biochemistry, immunology, molecular biology, biotechnology, and bio-nanotechnology in the past 20 years in the People’s Republic of China, Japan, and the U.S.A. Dr. Zhang was working on the development and detection of Hemorrhagic Fever vaccine and Japanese Encephalitis vaccine when she was in the Shanghai Institute of Biological product. She then received her Ph.D training on the mechanisms of enterobacterial infection and the development of a simple, rapid and relatively non-expensive diagnostic test to detect enterotoxins of Vibrio cholerae and enterotoxigenic Escherichia coli from clinical and environment samples. She has gained experiences on plant biotechnology, especially on gene mutation; expression and purification of mutant protein during her postdoctoral training at Michigan Tech. She has then engaged on bio-nanotechnology research on functionalization of carbon nanotubes with fluoresceinisothiocyanate (FITC) labeled bovine serum albumin (BSA). Her expertise includes bacteriology culture techniques, separation and identification pathogenic species of enteropathogenic bacterium, tissue culture, fluorescence microscopy, protein expression and purification, production of monoclonal antibody and polyclonal antibody, enzyme-linked immunosorbent assay (ELISA), immunoblotting, immunoprecipitation, immunohisto chemistry technique, isolation of DNA and RNA, PCR and RT-PCR, hybridization of DNA or RNA.

Appointments and Honors

• 2007- Adjunct Assistant Professor, Michigan Tech University, Houghton, MI, USA
• 2005-2006 Senior Research Scientist, Michigan Tech University, Houghton, MI, USA
• 2002-2005 Research Associate, Michigan Tech University, Houghton, MI, USA
• 1999-2002 Research Associate, Research Institute for Microbiology, Osaka University, Japan
• 1996-1997 Lions Club International Scholarship from Amagasaki Lions Club, Japan
• 1997-1999 Honors Scholarship from the Association of the International Education, Japan
• 1991-1994 Research Associate, Shanghai Institute of Biological Product, China

Professional Preparation and Experience

Selected Publications

1. S. Bhandari, T. Fujino, S. Thammanagowda, D. Zhang, F. Xu, and C. P. Joshi: Coordinate expression of tension stress-responsive and secondary cell wall-associated KORRIGAN endoglucanase and three cellulose synthase genes in aspen trees. Planta 224 (2006) 828-837.

2. D. Zhang, and T. Honda, “Glycophorin B is a receptor for Vibrio cholerae El Tor hemolysin”: In proceeding of the 7th China-Japan international congress of microbiology shanghai symposium-2000, 4-6 Aug, Shanghai, China (2000) 145.

3. D. Zhang, J. Takahashi, T. Seno, Y. Tani, and T.Honda, “Analysis of receptor for Vibrio cholerae El Tor hemolysin with a monoclonal antibody that recognizes glycophorin B of human erythrocyte membrane”, Infect. Immun. 67 (1999) 5332-5337.

4. N. Koike, Y. Yabushita, D. Zhang, T. Iida, and T. Honda, “Increased sensitivity of cholera toxin detection by enzyme-linked immunosorbent assay with chemical immobilization of antibody onto nylon surface providing hydrophobicity”, Microbiol. Immunol. 43 (1999) 1057-1060.

5. D. Zhang, and T. Honda, “Disappearance of glyceraldehyde 3-phosphate dehydrogenase from erythrocyte membrane by hemolysis with thermostable direct hemolysin of Vibrio parahaemolyticus or Vibrio cholerae El Tor hemolysin”, Microbiol. Immunol.43 (1999) 303-305.

6. D. Zhang, I. Yanagihara, T. Iida, and T. Honda, “Analysis of the El Tor hemolysin receptor with a monoclonal antibody that recognizes human erythrocyte membrane molecule”: In proceeding of the 34th joint conference U.S.- Japan cooperative medical science program cholera and other bacterial enteric infections panel, 30 Nov – 2 Dec, Shonan Village, Japan (1998) 159-163.

7. N. Koike, K. Okada, Y. Yabushita, D. Zhang, K. Yamamoto, T. Miwatani and T. Honda, “Rapid and differential detection of two analogous enterotoxins of Vibrio cholerae and enterotoxigenic Escherichia coli by a modified enzyme-linked immunosorbent assay”, FEMS Immunol. Med. Microbiol. 17 (1997) 21-25.